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Description

SIH MediumSIH medium, developed by Hanh, Friehs and Flaschel (2004), is the next step in the development of synthetic media designed to grow D. discoideum in high cell densities. SIH medium is an improved version of FM medium, as developed by Frank and Kessin (1977). The main difference between SIH and FM is the novel addition of Aspartic acid (1. 1 mM) and increased levels of Lysine (8. 5 mM) and Tryptophane (1. 7 mM). Concentrations of Cysteine, Glutamic

3 gram powdered medium in 1 litre distilled water

Process Biochemistry

Glutamic Acid

5x is a 5x concentrate and has to be diluted to a 1x concentration and subsequently supplemented with an appropriate carbon source

0002% Sulfate (SO4) <0

MOPS is frequently used as a buffering agent in biology and biochemistry

2 at 25°C Suspend 47

Chloramphenicol is a bacteriostatic antibiotic agent against gram-negative and gram-positive bacteria

coli cells

Potato extract is a mixture of potato proteins

50 X sterile stock solution

Colour Light cream Mesh Size (ASTM / Retained on Mesh 80): >50 on mesh 80 >90 on mesh 200 Gel Strength (Nikkan method) 700 - 1000 g/cm2 Moisture <15% pH (1

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